abstract：:A high-speed voltammetric system was designed and tested for dual measurement of dopamine (DA) and serotonin (5-HT) at 250-ms intervals. First, an anodic-cathodic square-wave pulse was delivered to activate the electrode (carbon fiber, 7 microm in diameter), then DA and 5-HT oxidation currents (current intensity) were...

journal_title：Journal of neuroscience methods

authors： Nakazato T,Akiyama A

更新日期：1999-07-15 00:00:00

abstract：:Organotypic brain slices cultured on semi-porous membranes is an increasingly popular in vitro preparation for studying mechanisms of ischemic brain damage. To model in vivo hypoxia, cultured brain slices are exposed to anaerobic atmosphere by placing them into a special incubator. This requirement limits the use of i...

journal_title：Journal of neuroscience methods

authors： Frantseva MV,Carlen PL,El-Beheiry H

更新日期：1999-07-01 00:00:00

abstract：:A microthermistor positioned close to the exposed posterior semicircular canal in isolated labyrinth preparations of the frog was used to stimulate the sensory organ. Our results indicated that, depending on the position of the heater, the induced endolymphatic convection currents may result in either excitatory or in...

journal_title：Journal of neuroscience methods

authors： Zucca G,Botta L,Valli S,Giannoni B,Mira E,Perin P,Valli P

更新日期：1999-05-01 00:00:00

abstract：:In the method of in situ hybridization autoradiography, quantitative comparisons among multiple mRNA signals have proven difficult for many reasons, attributable both to technical factors (e.g. different probe specific activities) as well as to large differences in the patterns and levels of expression of different ge...

journal_title：Journal of neuroscience methods

authors： Zhao W,Truettner J,Schmidt-Kastner R,Belayev L,Ginsberg MD

更新日期：1999-04-01 00:00:00

abstract：:The object-oriented graphical programming language LabView was used to implement the numerical solution to a computational model of saccade generation in primates. The computational model simulates the activity and connectivity of anatomical strictures known to be involved in saccadic eye movements. The LabView progra...

journal_title：Journal of neuroscience methods

authors： Jackson ME,Gnadt JW

更新日期：1999-03-01 00:00:00

abstract：:Using a specific rat monoclonal anti-idiotypic antibody we have examined the subcellular distribution of -opioid receptors in various neuronal subtypes of the rat spinal cord. The immunofluorescence was detected with a confocal microscope and in some cases serial images were processed for a three-dimensional (3-D) rec...

journal_title：Journal of neuroscience methods

authors： Mailly P,Gastard M,Cupo A

更新日期：1999-02-01 00:00:00

abstract：:A reliable and economical silver staining method is recommended for demonstration of Lewy bodies (LB) and Lewy neurites (LN) in Parkinson's disease (PD) and of argyrophilic oligodendrocytes in multiple system atrophy (MSA). The technique can be applied to routinely formalin-fixed autopsy material and does not require ...

journal_title：Journal of neuroscience methods

authors： Braak E,Braak H

更新日期：1999-02-01 00:00:00

abstract：:This review considers the different forms of reversible deactivation and how they differ from each other and from more conventional permanent deactivation methods. The different methods of chemical and cooling reversible inactivation and the limitations of each are discussed. Finally, future directions in the quest to...

journal_title：Journal of neuroscience methods

authors： Lomber SG

更新日期：1999-01-01 00:00:00

abstract：:We present a high-speed, high-resolution imager of beta particles. It is devoted to be used in autoradiography experiments such as receptor binding or in situ hybridization experiments, either instead of, or in complement with autoradiographic film and emulsions. It allows the user to locate and perform quantitative a...

journal_title：Journal of neuroscience methods

authors： Laniece P,Charon Y,Cardona A,Pinot L,Maitrejean S,Mastrippolito R,Sandkamp B,Valentin L

更新日期：1998-12-31 00:00:00

abstract：:A reliable source of human neural tissue would be of immense practical value to both neuroscientists and clinical neural transplantation trials. In this study, human precursor cells were isolated from the developing human cortex and, in the presence of both epidermal and fibroblast growth factor-2, grew in culture as ...

journal_title：Journal of neuroscience methods

authors： Svendsen CN,ter Borg MG,Armstrong RJ,Rosser AE,Chandran S,Ostenfeld T,Caldwell MA

更新日期：1998-12-01 00:00:00

abstract：:The incidence of leptomeningeal carcinomatosis is on the rise and current treatment modalities have limited efficacy. The development of new treatment strategies has been hampered by the lack of an appropriate animal model that accurately parallels the clinical condition. We have developed a new surgical technique for...

journal_title：Journal of neuroscience methods

authors： Janczewski KH,Chalk CL,Pyles RB,Parysek LM,Unger LW,Warnick RE

更新日期：1998-11-01 00:00:00

abstract：:Biolistics, also known as particle-mediated gene transfer, has been used as an effective, method to transfect primary neurons in cultured slices when all other methods have proven unsuccessful. Most of these uses have provided qualitative or semi-quantitative data based on visual assays such as immunohistochemistry. I...

journal_title：Journal of neuroscience methods

authors： Thomas A,Kim DS,Fields RL,Chin H,Gainer H

更新日期：1998-10-01 00:00:00

abstract：:Microglia are brain cells of considerable interest because of their role in CNS inflammatory responses and strong association with neuritic plaques in Alzheimer's disease (AD). In the present study, immunocytochemistry was combined with unbiased stereology to estimate the mean total number of microglia in dentate gyru...

journal_title：Journal of neuroscience methods

authors： Long JM,Kalehua AN,Muth NJ,Hengemihle JM,Jucker M,Calhoun ME,Ingram DK,Mouton PR

更新日期：1998-10-01 00:00:00

abstract：:A common approach to test the time-response of carbon fibre microelectrodes entails the use of a flow injection system which allows delivery of a bolus of test compound in the flow stream to a detector electrode. The introduced bolus is distorted from its original form by convective and dispersive forces during the pe...

journal_title：Journal of neuroscience methods

authors： Yavich L

更新日期：1998-10-01 00:00:00

abstract：:We investigated the usefulness of the Overall Rotarod Performance (ORP) test for evaluating overall locomotory ability in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-injected-mouse model of Parkinson's disease (PD). For this procedure, the mice are pretrained on the rotarod and then tested at a series of i...

journal_title：Journal of neuroscience methods

authors： Rozas G,López-Martín E,Guerra MJ,Labandeira-García JL

更新日期：1998-09-01 00:00:00

abstract：:The last decade has seen a surge in the study of nonlinear dynamical behavior in physiologic systems. In this paper, some of the computational techniques most commonly used to investigate the nonlinear dynamics of these systems are described. Applications to eye movement analysis are included, including the validation...

journal_title：Journal of neuroscience methods

authors： Shelhamer M

更新日期：1998-08-31 00:00:00

abstract：:Analysis and interpretation of current records from cellular membranes or lipid bilayers is complicated by perturbations. These perturbations can originate from random noise or deterministic artifacts as capacitive currents caused by step depolarization's, drift, 50 Hz pick up or microphonics. Accurate removal of dete...

journal_title：Journal of neuroscience methods

authors： Baumgartner W,Schindler H,Romanin C

更新日期：1998-08-01 00:00:00

abstract：:The Utah Intracortical Electrode Array (UIEA) is an array of 100 penetrating silicon microelectrodes designed to focally electrically stimulate or record neurons residing in a single layer up to 1.5 mm beneath the surface of the cerebral cortex. Apart from its use as a unique tool to study parallel processing in the c...

journal_title：Journal of neuroscience methods

authors： Rousche PJ,Normann RA

更新日期：1998-07-01 00:00:00

abstract：:A critical problem in neuroscience is the lack of database tools for integrating neuronal property data. We report here the development of a combined object oriented-relational database (NeuronDB, http://senselab.med.yale.edu/neurondb) that meets these needs by providing tools for integrating data within neurons and c...

journal_title：Journal of neuroscience methods

authors： Mirsky JS,Nadkarni PM,Healy MD,Miller PL,Shepherd GM

更新日期：1998-07-01 00:00:00

abstract：:We have designed a microdialysis technique to measure acetylcholinesterase (AChE) activity in the cortex of freely moving rats while simultaneously measuring the release of acetylcholine (ACh). Our approach was validated using ethyl S-2-di-isopropylaminoethyl-phosphonothiolate (VX), an irreversible inhibitor of AChE a...

journal_title：Journal of neuroscience methods

authors： Testylier G,Micoud N,Martinez S,Lallement G

更新日期：1998-06-01 00:00:00

abstract：:The naked mole-rat (Heterocephalus glaber) is a unique fossorial mammal that is eusocial, object blind, and virtually poikilothermic. This animal is an ideal model to examine questions pertaining to the thermoregulatory aspects of sleep/wake cycles and other circadian events. However, the monitoring of mammalian sleep...

journal_title：Journal of neuroscience methods

authors： Herold N,Spray S,Horn T,Henriksen SJ

更新日期：1998-06-01 00:00:00

abstract：:A computational model has been developed for the action potential and, more generally, the electrical behaviour of the rat sympathetic neurone. The neurone is simulated as a complex system in which five voltage-dependent conductances (gNa, gCa, gKV, gA, gKCa), one Ca2+-dependent voltage-independent conductance (gAHP) ...

journal_title：Journal of neuroscience methods

authors： Sacchi O,Belluzzi O,Canella R,Fesce R

更新日期：1998-04-30 00:00:00

abstract：:Event-related potentials (ERPs) are evoked brain potentials that are averaged across many trial repetitions with individual trials aligned (i.e. time-locked) to a specific behavioral event, typically the onset of the stimulus (s-lock) or the onset of the behavioral response (r-lock). These evoked potential averages ma...

journal_title：Journal of neuroscience methods

authors： Zhang J

更新日期：1998-03-13 00:00:00

abstract：:Spike-triggered averaging of EMG is a useful experimental technique for revealing functional connectivity from central neurons to motoneurons. Because EMG waveforms constitute time series, statistical analysis of spike-triggered averages is complicated. Empirical methods generally have been employed to detect the pres...

journal_title：Journal of neuroscience methods

authors： Poliakov AV,Schieber MH

更新日期：1998-02-20 00:00:00

abstract：:Morphometric estimation of neuronal processes is currently laborious and time-consuming, since the individual processes (axons and dendrites) have to be traced manually. In order to facilitate the measurement of cellular processes, we have tested a series of parameters derived from the contour and the convex hull of a...

journal_title：Journal of neuroscience methods

authors： Kawa A,Stahlhut M,Berezin A,Bock E,Berezin V

更新日期：1998-01-31 00:00:00

abstract：:Both extracellular and intracellular stimulation of single motoneurons were shown to be similarly effective and consistent in eliciting contractile responses in single lateral rectus muscle motor units. The whole muscle was activated by stimulating the sixth nerve in the brain stem. Both whole muscle and motor unit co...

journal_title：Journal of neuroscience methods

authors： Goldberg SJ,Shall MS

更新日期：1997-12-30 00:00:00

abstract：:The present study evaluated high-speed chronoamperometry as a method for measuring the clearance of serotonin (5-HT) from extracellular space in vivo. Male Sprague-Dawley rats were anaesthetized and a Nafion-coated, carbon fiber electrode, attached to a multibarrel pipette, was lowered into the subgranular layer of th...

journal_title：Journal of neuroscience methods

authors： Daws LC,Toney GM,Davis DJ,Gerhardt GA,Frazer A

更新日期：1997-12-30 00:00:00

abstract：:A sensitive, enzymatic glucose electrode was coupled with the microdialysis sampling technique to enable the continuous, on-line measurement of dialysate glucose. The glucose sensitive electrode was fabricated by immobilizing glucose oxidase onto the surface of an osmium-polyvinylpyrridine horse radish peroxidase gel ...

journal_title：Journal of neuroscience methods

authors： Osborne PG,Niwa O,Kato T,Yamamoto K

更新日期：1997-12-01 00:00:00

abstract：:Immunodetection techniques are dependent on enzyme-protein conjugates for the visualisation of antigen-antibody complexes. One of the most widely used is the avidin-biotin-peroxidase complex (ABC) method. The present study demonstrates that direct treatment of goldfish and salamander retinal sections with ABC, followe...

journal_title：Journal of neuroscience methods

authors： Bhattacharjee J,Nunes Cardozo B,Kamphuis W,Kamermans M,Vrensen GF

更新日期：1997-11-07 00:00:00

abstract：:We have examined the stability and sources of variation within the nociceptive model of rat hind paw withdrawal from an under-glass radiant stimulus (Hargreaves et al., 1988) using a system where stimulus intensity and floor temperature can be controlled and reproducibly changed. The current study demonstrates that: (...

journal_title：Journal of neuroscience methods

authors： Dirig DM,Salami A,Rathbun ML,Ozaki GT,Yaksh TL

更新日期：1997-10-03 00:00:00

abstract：:We describe a method for optically recording neuronal activity from an intact insect brain, upon natural sensory stimulation. In this preparation, the head capsule of the honeybee, Apis mellifera, is isolated from the body while leaving the entire brain undamaged. In short, a hole is first cut into the cuticule to all...

journal_title：Journal of neuroscience methods

authors： Galizia CG,Joerges J,Küttner A,Faber T,Menzel R

更新日期：1997-09-05 00:00:00

abstract：:Binding of the cocaine analog 3 beta-(4-[125I]iodophenyl)tropane-2 beta-carboxylic acid isopropyl ester ([125I]RTI-121) to filters was studied in order to assess its contribution to labeling dopamine transporters on rat striatal synaptosomal membranes in filtration assays. Filter binding (FB) decreased with increasing...

journal_title：Journal of neuroscience methods

authors： Chen NH,Ding JH,Wang YL,Reith ME

更新日期：1997-08-22 00:00:00

abstract：:Genetic intervention is a powerful means for answering basic biological questions about molecular events taking place in brain cells underlying complex behaviors. Traditional transfection method has proved difficult to apply to neuronal cells. We therefore described an alternative procedure for introducing DNA into cu...

journal_title：Journal of neuroscience methods

authors： Li H,Chan ST,Tang F

更新日期：1997-07-18 00:00:00

abstract：:A simple method for making a stimulating multi-channel metallic electrode is proposed. The method uses furnace-shaped multiple fine glass tubes as channel guides for inserted tungsten wires that are pulled as a unit to produce multi-channel metallic electrode with a tapered tip. By shortening the tip, the desired inte...

journal_title：Journal of neuroscience methods

authors： Kuras A,Gutmaniene N

更新日期：1997-07-18 00:00:00

abstract：:Human muscle fatigue has been studied using a wide variety of exercise models, protocols and assessment methods. Based on the definition of fatigue as 'any reduction in the maximal capacity to generate force or power output', the different methods to measure fatigue are discussed. It is argued that reliable and valid ...

journal_title：Journal of neuroscience methods

authors： Vøllestad NK

更新日期：1997-06-27 00:00:00

abstract：:The permanence of retrograde neuronal labeling by the fluorescent tracers Fast Blue, Fluoro-Gold, Mini-Ruby, Fluoro-Ruby and Fluoro-Emerald was investigated in adult rat spinal motorneurons at 1, 4, 12 and 24 weeks after tracer application to a transected muscle nerve. After 1 week, the largest number of retrogradely ...

journal_title：Journal of neuroscience methods

authors： Novikova L,Novikov L,Kellerth JO

更新日期：1997-06-06 00:00:00

abstract：:In order to optimize a method for quantitative assessment of bradykinesia, we evaluated the three-dimensional sources of a movement signal of the wrist and influence of tremor on the reliability of bradykinesia measurements. A total of 33 patients with Parkinson's disease, three patients with Multiple System Atrophy a...

journal_title：Journal of neuroscience methods

authors： Dunnewold RJ,Jacobi CE,van Hilten JJ

更新日期：1997-06-06 00:00:00

abstract：:Synthesis of melatonin in the mammalian pineal gland is regulated by the rhythmic expression of acetyl-CoA: serotonin N-acetyltransferase (SNAT) and other unknown factors. To screen for pineal-specific mRNAs potentially involved in melatonin synthesis and/or regulation, differential display PCR (DDPCR) was employed. W...

journal_title：Journal of neuroscience methods

authors： Wang X,Brownstein MJ,Young WS

更新日期：1997-05-16 00:00:00

abstract：:Recently there has been an increase in the use of spectral methods for the analysis of experimental data. These analytical methods allow the study of interactions between simultaneously recorded signals and are particularly suited to the study of systems displaying rhythmic behaviour. A useful parameter in this contex...

journal_title：Journal of neuroscience methods

authors： Amjad AM,Halliday DM,Rosenberg JR,Conway BA

更新日期：1997-04-25 00:00:00

abstract：:Measurements of neurotransmitters in conscious animals have been restrictive in real-time. The use of specific enzymes within an amperometric probe, based upon a microdialysis membrane, may overcome some of these problems. I report the use of such a probe, with different perfusions of enzymes, to allow real-time measu...

journal_title：Journal of neuroscience methods

authors： Cook CJ

更新日期：1997-04-04 00:00:00